Alpha Omega Alpha Honor Medical Society

2013 Research Abstract

Aquaporin 4 Expression in Astrocytes Subjected to an In Vitro Model for Ischemia

Investigator: Nancy Glober

Mentor: Murat Digicaylioglu, MD, PhD

Abstract: Edema secondary to Traumatic Brain Injury (TBI) causes morbidity and mortality. Aquaporin 4 (AQP4) is a water channel expressed in the central nervous system (CNS) that regulates edema formation, however, the regulation of AQP4 in the CNS remains poorly understood. We show that AQP4 mRNA increases in cultured astrocytes after 12 h of oxygen-glucose deprivation (OGD), whereas AQP4 protein increases after 48 h of OGD. In addition, we show that miRNA-210 may be involved in the regulation of AQP4 mRNA. AQP4 membrane distribution in astrocytes subjected to OGD changed from a diffuse distribution to a clustered one. However, astrocytes that were mechanically stretched did not show the same distribution pattern. Treatment with Acetazolamide inhibited the changes in distribution of AQP4 caused by OGD, and mice treated with Acetazolamide after TBI demonstrated less cytotoxic edema than controls, suggesting a possible clinical role for Acetazolamide in the treatment of TBI.

Last updated: 10/2/2014

Updated on September 2, 2014.

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