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2010 Research Abstract

Receptor selection in mouse bone marrow B cells

Investigator: Karen Li, Drexel University College of Medicine
Mentor: Nina Luning Prak, MD, PhD, University of Pennsylvania School of Medicine

Aberrant light chain rearrangement has been implicated in the loss of tolerance in self-reactive B cells. Ongoing light chain rearrangement, a process termed receptor editing, is a major mechanism for tolerizing self-reactive B cells. We have observed that highly edited B cells become less frequent once fully assembled antibody molecules are expressed on the B cell surface. We hypothesized that the decrease in highly edited B cells is due either to negative selection (death of cells that have failed to edit successfully), positive selection of cells with fewer rearrangements or escape of edited B cells to a hitherto unidentified peripheral compartment. Here, we report preliminary data from a pilot experiment that macrophages may be involved in the selective depletion of highly edited B cells. Flow cytometry analysis revealed co-expression of macrophage markers, F4/8 and CD-11, in anti-DNA heavy chain knock-in mouse (B6.56.R) bone marrow. Co-staining analysis with early B cell markers for anti-CD-19 and CD-11 showed that most bone marrow cells positive for macrophage markers were also positive for early B cell markers. Taken together, our results may provide a clue that macrophages may be involved in the clearance of highly edited B cells.

Updated on June 8, 2011.